Expression of the von Willebrand factor gene is tissue specific and confined to endothelial cells and megakaryocytes. VWF is present in plasma, in the Weibel-Pallade bodies of endothelial cells, in the alpha-granule in megakaryocytes and platelets derived from them, as well as in the subendothelial matrix of the vessel wall. Results aid in the classification of acute myeloid leukemia FAB type M7, angiosarcoma and epithelioid hemangioendothelioma.
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
Enzyme/HIER
Clone
Polyclonal
Code Number
A0082
Immunogen
Von Willebrand factor isolated from human plasma.
Reagent Provided
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
Solutions
Concentrate
Species
Rabbit Anti-Human
Specificity
The antibody reacts with human von Willebrand factor/factor VIII complex. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. Polyclonal Rabbit Anti-Human Von Willebrand Factor reacts with von Willebrand factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) (7). See package insert for reference(s).
In crossed immunoelectrophoresis using 12.5 µL antibody per cm2 gel area against 10 µL of human plasma only one precipitate corresponding to the von Willebrand factor appears. Staining: Coomassie Brilliant Blue.
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Weidner N, Semple JP, Welch WR, Folkman J. Tumor angiogenesis and metastasis - correlation in invasive breast carcinoma. N Engl J Med 1991,324:1-8. 2. Makhlouf HR, Ihsak KD, Goodman ZD. Epithelioid hemangioendothelioma of the liver: a clinicopathologic study of 137 cases. Cancer 1999,85:562-82.