Tyrosinase is a copper-glycoenzyme involved in the production of melanin pigments, including both eumelanin and pheomelanin. As a marker of melanocytic lineage, tyrosinase is localized to melanocytes which can be found on the dermal/epidermal junction of normal skin. It is not detected in other normal cells. Results aid in the classification of primary and metastatic malignant melanomas. The cellular localization of tyrosinase is cytoplasmic and/or perinuclear.
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Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Purified recombinant unglycosylated tyrosinase protein consisting of 452 amino acids of the 511 amino acid mature human tyrosinase protein (1). See package insert for reference(s).
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Autostainer Link 48
Immunoblotting was performed using L-cells transfected with the tyrosinase gene and on four cell lines that express tyrosinase mRNA: SK-MEL-13, SK-MEL-19, SK-MEL-30 and SK-MEL-37 (1). Consistent with previous reports, a cluster of proteins ranging from 70-80 kDa was recognized by clone T311 in immunoblots of all five cell lines (1). An additional 55 kDa band was stained in blots from cell lines that are strongly reactive (SK-MEL-19) (1). Furthermore, immunoblots of untransfected L-cells and three cell lines that do not express tyrosinase mRNA (MZ2-MEL3.1, SK-MEL-187 and MZ2-MEL2.2) were negative with clone T311 (1). L-cells transfected with the tyrosinase-related protein 1, TRP-1(gp75), were also unreactive with clone T311 in immunofluorescence and immunoblotting assays, indicating this antibody does not cross-react with the melanosome-associated protein, TRP-1(gp75) (1). See package insert for reference(s).