Lung

Thyroid Transcription Factor (Autostainer Link 48)

Clone 8G7G3/1

Identifies the 40 kDa TTF-1 band in nuclear extracts or whole cell lysates of TTF-1-positive cell lines of rat, mouse and man. TTF-1 is selectively expressed in lung and thyroid, and the antibody may aid in the classification of tumors of the lung and thyroid.

Monoclonal Mouse Anti-

Features

Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.

Specifications


Application	Formalin HIER
Clone	8G7G3/1
Code Number	IR056
Immunogen	Recombinant rat TTF-1 (1). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN₃.
Solutions	Autostainer Link 48
Species	Mouse Anti-
Specificity	Monoclonal anti-rat TTF-1 clone 8G7G3/1 (anti-TTF-1) has been shown to react with human TTF-1. In Western blotting, anti-TTF-1 specifically identified a 40 kDa band in immunoblots of nuclear extracts or whole cell lysates from the TTF-1 positive cell lines MLE 15 (mouse lung epithelial), H441-4 (human lung adenocarcinoma) and H 345 (human small cell carcinoma). Cell lines lacking TTF-1 mRNA such as HeLa and 3T3 were unreactive. Anti-TTF-1 was also found to function in ELISA assays using recombinant TTF-1 (1). See package insert for reference(s).

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