Reacts strongly with human S100B, and weakly or very weakly with S100A1 and S100A6, respectively. S100 from ox brain has been used for the immunization. Z0311 labels glial cells in the brain and ependymal cells. Moreover, Schwann's cells of the peripheral nervous system are positive. Results aid in the classification of tumors in central and peripheral nervous system, such as schwannomas, ependydomas as well as in different grades of astrogliomas, also including glioblastomas. A large proportion of cells in human tumors originating from different salivary glands are labeled by anti-S100. The antibody is also a useful aid for classification of malignant melanocytic tumors of the skin and metastases of human malignant melanomas.
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Product Details
Features
Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Specifications
Application
Formalin
HIER
Clone
Polyclonal
Code Number
IR504
Immunogen
S100 isolated from cow brain.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions
Autostainer Link 48
Species
Rabbit Anti-
Specificity
The antibody has been solid-phase absorbed with human plasma and cow serum proteins. In Western blotting of purified human recombinant S100 proteins, the antibody labels S100B strongly, S100A1 weakly, and S100A6 very weakly. No reaction was observed with the other S100 proteins tested, S100A2, S100A3 and S100A4 (8). In indirect ELISA, the antibody shows no reaction with human plasma and cow serum. As demonstrated by IHC on formalin-fixed, paraffin-embedded tissue sections, the antibody cross-reacts with the S100 equivalent protein in man. See package insert for reference(s).