PTEN Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	6H2.1
Code Number	M3627
Immunogen	The last 100 C-terminal amino acids of PTEN (Ziebold and Lees, unpublished) (15). See package insert for reference(s).
Isotype	IgG2a, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and 0.015 mol/L sodium azide. Contains stabilizing protein.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	Monoclonal mouse anti-PTEN clone 6H2.1, identifies a single band at the 55 kDa predicted molecular weight for PTEN in Western blots of MCF-7, T-47D and MDA-MB-435S PTEN homozygous cell lines. PTEN null cell lines BT-549 and MDA-MB-468 cell lines were unreactive with clone 6H2.1 by Western blot analysis. Immunoblots of MCF-7 cells induced to express PTEN demonstrated an increased band intensity at the expected size while a weak band was observed on immunoblots of ZR-75-1 cell lysates containing a hemizygous deletion of PTEN and a mis-sense mutation in the remaining allele (15). Immunohistochemical staining of paraffin-embedded cell blocks of Ishikawa 3-H-12 cells transfected with PTEN stained positively whereas PTEN deficient 3-H-12 cell block sections were unreactive (1). See package insert for reference(s).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Featured References

Product Description References: 1. Perren A, Weng LP, Boag AH, Ziebold U, Thakore K, Dahia PL, et al. Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast. Am J Pathol 1999,155:1253-60.
2. McMenamin ME, Soung P, Perera S, Kaplan I, Loda M, Sellers WR. Loss of PTEN expression in paraffin-embedded primary prostate cancer correlates with high Gleason score and advanced stage. Cancer Res 1999,59:4291-6.

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PTEN (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

View supported Instructions For Use documents

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