Benign prostatic hyperplasia

Prostein (Concentrate)

Clone 10E3

Prostein is a 553-amino acid protein, also known as P501S. Prostein protein is a type IIIa plasma membrane protein which has been shown to be exclusively expressed in cells of normal and malignant prostate by Northern blot, cDNA microarray, real-time PCR and immunohistochemistry. Prostein is localized to the Golgi complex in the cytoplasm of cells and is expressed by both benign and neoplastic prostate tissue, whereas it has not been detected in any other normal or malignant tissue examined.

This antibody is a useful aid for classification of prostate adenocarcinomas.

Monoclonal Mouse Anti-Human

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	10E3
Code Number	M3615
Immunogen	Recombinant truncated prostein, containing amino acids 341-527 (1). See package insert for reference(s).
Isotype	IgG2a, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 0.015 mol/L sodium azide. This product contains stabilizing protein.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	Monoclonal mouse anti-prostein has been demonstrated to react with a 21 kDa recombinant prostein fragment and with a 50 kDa doublet present in Western blots of prostein-transfected HEK293 cell lysates. Anti-prostein also detected a 50 kDa doublet in blots of LNCaP and PC3 prostate tumor cell lysates, whereas the antibody was unreactive on Western blots of prostein negative Du145 cells and untransfected HEK293 cells (1). The epitope recognized by anti-prostein was mapped using a series of overlapping peptides spanning the amino acid sequence of the anti-prostein immunogen. Using ELISA, the epitope recognized by anti-prostein was localized to amino acids 439-459 of the prostein sequence (1). See package insert for reference(s).

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Prostein (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

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