Papillomavirus Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
For immunohistochemistry (IHC)
Certified manufacturing facilities guarantee full quality control
Dako products are the result of almost 50 years experience ensuring quality and innovation in antibodies and reagents: the genesis of the pathology lab of tomorrow.

Specifications


Application	Frozen Formalin HIER
Clone	K1H8
Code Number	M3528
Immunogen	Alkaline disrupted HPV type 1 (2). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	Anti-human papillomavirus, clone K1H8 (anti-HPV) reacts with a non-conformational internal linear epitope of a major capsid protein of HPV-1, which is broadly expressed among the different HPV subtypes. In Western blotting, anti-HPV identified a 57 kD protein, which corresponds to the molecular weight of a major polypeptide of the capsid protein VP1. Minor bands of 47, 44, 41 and 33 kD were weakly labeled and thought to be degraded capsid proteins. Anti-HPV has been demonstrated to identify HPV-1 antigen in ELISA and to detect HPV-1 and HPV-6 by indirect immunofluorescence in infected frozen tissue sections. Anti-HPV was found to be immunoreactive with paraffin sections of formalin-fixed HPV-infected tissues which were demonstrated by Southern blot hybridization to be infected with HPV type 6, 11, 16, 18, 31, 33, 42, 51, 52, 56 and 58. Positive immunostaining was largely confined to the nuclei of infected cells. Occasionally, the cytoplasm of koilocytotic cells was observed to be immunoreactive (2). See package insert for reference(s).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Papillomavirus (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

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