Squamous cell carcinoma

p53 Protein (Autostainer Link 48)

Clone DO-7

Reacts with the wild type and mutant type of the p53 protein. The antibody is a useful aid for classification of tumors of all cell lineages.

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.

Specifications


Application	Formalin HIER
Clone	DO-7
Code Number	IR616
Immunogen	Recombinant human wild-type p53 protein (1). See package insert for reference(s).
Isotype	IgG2b, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Autostainer Link 48
Species	Mouse Anti-Human
Specificity	In Western blotting of lysate of the A431 human vulval carcinoma cell line, the antibody labels a 53 kDa band, corresponding to the mutant type p53, which is expressed by A431. The epitope recognized by the antibody is located between the N-terminal amino acids 1 and 45 and possibly between amino acids 37 and 45 of the human p53 protein (1). SDS-PAGE analysis of immunoprecipitates formed between lysate of the BT474 breast cancer cell line and the antibody shows reaction with a 53 kDa protein corresponding to p53 (1). In IHC, the antibody labels mutant-type p53 in the A431 cell line and wild-type p53 in the SVK14 cell line (SV40-transformed keratinocyte line) (1). See package insert for reference(s).

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