Embryonal carcinoma

Octamer-Binding Transcription Factor 3/4 (Concentrate)

Clone N1NK

Octamer-binding transcription factor 3/4 (OCT3/4) is expressed in early embryonic cells and germ cells and is central to the gene regulatory network responsible for self-renewal, pluripotency, and lineage commitment in embryonic stem cells and induced pluripotent stem cells (1).

Antibodies to OCT3/4 may be a useful aid for classification of specific subtypes of germ cell tumors including seminoma, embryonal carcinoma and intratubular germ cell neoplasia of unclassified type (IGCNU) (2, 3).

Monoclonal Mouse Anti-Human

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	N1NK
Code Number	M3649
Immunogen	Recombinant protein corresponding to 147 amino acids at N-terminus of human OCT3/4.
Isotype	IgG1, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as cell culture supernatant in 0.05 mol/L Tris-HCl, 0.015 mol/L sodium azide, pH 7.2. This product contains stabilizing protein.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	In Western blotting of hES-TW1 cell lysates, Anti-OCT3/4, clone N1NK recognizes a major band of ~40 kDa corresponding to the expected molecular weight of OCT3/4 (9). See package insert for reference(s).

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Featured References

Product Description References: 1. Bhartiya D, Kasiviswanathan S, Unni SK, Pethe P, Dhabalia J, Patwardhan S, et al. Newer insights into premeiotic development of germ cells in adult human testis using Oct-4 as a stem cell marker. J Histochem Cytochem 2010,58:1093-1106.
2. Cheng L. Establishing a germ cell origin for metastatic tumors using OCT4 immunohistochemistry. Cancer 2004,101:2006-10.
3. Jones TD, Ulbright TM, Eble JN, Cheng L. OCT4: a sensitive and specific biomarker for intratubular germ cell neoplasia of the testis. Clin Cancer Res 2004,10:8544-7.

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Octamer-Binding Transcription Factor 3/4 (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

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