MUM1 Protein (Dako Omnis)

Clone MUM1p

Labels MUM1 protein in a subset of light zone germinal centre (GC) B cells (probably centrocytes and their progeny), plasma cells and activated T cells. Antibodies to MUM1 protein are a useful aid for classification of a wide spectrum of hematolymphoid neoplasms derived from these cells. Of non-hematolymphoid neoplasms only a proportion of melanomas are labeled (1, 2). Antibodies to MUM1 protein are a useful aid for subclassification of B-cell lymphomas (1-3).

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.

Specifications


Application	Formalin HIER
Clone	MUM1p
Code Number	GA644
Immunogen	Recombinant MUM1 fusion protein (1). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Dako Omnis
Species	Mouse Anti-Human
Specificity	Western blotting of pHeBo-CMV-MUM1-HA-transfected HeLa cell lysate, the antibody labels a band of 52 kDa corresponding to MUM1-HA protein, whereas no labeling is observed in non-transfected HeLa cell lysate. In Western blotting of lysates of IM9 (myeloma cell line), unfractionated tonsil cell suspensions, and PHA-stimulated T cells, the antibody labels a band of 50 kDa corresponding to MUM1 protein. No labeling is observed with lysates of U937 cells (myeloid cell line), and unstimulated T cells (1). SDS-PAGE analysis of immunoprecipitates formed between lysate of the IM9 myeloma cell line and the antibody shows reaction with a 50 kDa protein corresponding to MUM1 protein (1). See package insert for reference(s).

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Featured References

Product Description References: 1. Falini B, Fizzotti M, Pucciarini A, Bigerna B, Marafioti T, Gambacorta M, et al. A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells. Blood 2000,95,2084-92.
2. Natkunam Y, Warnke RA, Montgomery K, Falini B, van de Rijn M. Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry. Mod Pathol 2001,14:686-94.
3. Gaidano G, Carbone A. MUM1: a step toward the understanding of lymphoma histogenesis. Leukemia 2000,14:563-6.

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MUM1 Protein (Dako Omnis)

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