Labels MUM1 protein in a subset of light zone germinal centre (GC) B cells (probably centrocytes and their progeny), plasma cells and activated T cells. Antibodies to MUM1 protein are a useful aid for classification of a wide spectrum of hematolymphoid neoplasms derived from these cells. Of non-hematolymphoid neoplasms only a proportion of melanomas are labeled (1, 2). Antibodies to MUM1 protein are a useful aid for subclassification of B-cell lymphomas (1-3).
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Frozen
Formalin
HIER
Clone
MUM1p
Code Number
M7259
Immunogen
Recombinant MUM1 fusion protein (1). See package insert for reference(s).
Isotype
IgG1, kappa
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
Solutions
Concentrate
Species
Mouse Anti-Human
Specificity
In Western blotting of pHeBo-CMV-MUM1-HA-transfected HeLa cell lysate, the antibody labels a band of 52 kDa corresponding to MUM1-HA protein, whereas no labeling is observed in non-transfected HeLa cell lysate. In Western blotting of lysates of IM9 (myeloma cell line), unfractionated tonsil cell suspensions, and PHA-stimulated T cells, the antibody labels a band of 50 kDa corresponding to MUM1 protein. No labeling is observed with lysates of U937 cells (myeloid cell line), and unstimulated T cells (1). SDS-PAGE analysis of immunoprecipitates formed between lysate of the IM9 myeloma cell line and the antibody shows reaction with a 50 kDa protein corresponding to MUM1 protein (1). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Falini B, Fizzotti M, Pucciarini A, Bigerna B, Marafioti T, Gambacorta M, et al. A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells. Blood 2000,95,2084-92. 2. Natkunam Y, Warnke RA, Montgomery K, Falini B, van de Rijn M. Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry. Mod Pathol 2001,14:686-94. 3. Gaidano G, Carbone A. MUM1: a step toward the understanding of lymphoma histogenesis. Leukemia 2000,14:563-6.