Labels MUM1 protein in a subset of light zone germinal centre (GC) B cells (probably centrocytes and their progeny), plasma cells and activated T cells. Antibodies to MUM1 protein are a useful aid for classification of a wide spectrum of hematolymphoid neoplasms derived from these cells. Of non-hematolymphoid neoplasms only a proportion of melanomas are labeled (1, 2). Antibodies to MUM1 protein are a useful aid for subclassification of B-cell lymphomas (1-3).
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Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Recombinant MUM1 fusion protein (1). See package insert for reference(s).
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Autostainer Link 48
Western blotting of pHeBo-CMV-MUM1-HA-transfected HeLa cell lysate, the antibody labels a band of 52 kDa corresponding to MUM1-HA protein, whereas no labeling is observed in non-transfected HeLa cell lysate. In Western blotting of lysates of IM9 (myeloma cell line), unfractionated tonsil cell suspensions, and PHA-stimulated T cells, the antibody labels a band of 50 kDa corresponding to MUM1 protein. No labeling is observed with lysates of U937 cells (myeloid cell line), and unstimulated T cells (1). SDS-PAGE analysis of immunoprecipitates formed between lysate of the IM9 myeloma cell line and the antibody shows reaction with a 50 kDa protein corresponding to MUM1 protein (1). See package insert for reference(s).
1. Falini B, Fizzotti M, Pucciarini A, Bigerna B, Marafioti T, Gambacorta M, et al. A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells. Blood 2000,95,2084-92. 2. Natkunam Y, Warnke RA, Montgomery K, Falini B, van de Rijn M. Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry. Mod Pathol 2001,14:686-94. 3. Gaidano G, Carbone A. MUM1: a step toward the understanding of lymphoma histogenesis. Leukemia 2000,14:563-6.