MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein and is a useful aid for classification of primary and metastatic epithelioid malignant melanomas.
This product is not available for purchase by the general public.
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Histidine fusion protein of the amino-terminal Taq-Sac fragment of human MITF cDNA (1,2). See package insert for reference(s).
Monoclonal mouse antibody provided in liquid form as tissue culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and 0.015 mol/L sodium azide. Contains stabilizing protein.
In Western blot anaylsis of human melanoma cell line, 501mel, anti-MITF demonstrated 54 and 60 kDa bands corresponding to MITF variants differing in phosphorylation status. Further, specificity was demonstrated in gel-mobility assays where anti-MITF produced a specific gel-mobility supershift with MITF but not with related bHLHzip factors TFEB, TFEC and TFE3 (1). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.