Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas and angiomyolipomas. The antibody is a useful aid for classification of melanomas and adrenocortical carcinomas. The antibody is also a useful aid for classification of angiomyolipomas.
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Recombinant protein expressed in E. coli corresponding to Melan-A (1). See package insert for reference(s).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
In Western blotting of cell lysates from Melan-A mRNA positive cell lines, the antibody labels a protein doublet of 20-22 kDa, whereas no labeling is detected in Melan-A mRNA-negative cell lines (1). In immunoprecipitates of Melan-A positive cell lines SK-MEL-13 and SK-MEL-19, the antibody labels a protein doublet of 20-22 kDa corresponding to Melan-A, whereas no precipitation is detected in Melan-A mRNA-negative melanoma cell lines (1). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Fetsch PA, Cormier J, Hijazi YM. Immunocytochemical detection of MART-1 in fresh and paraffin-embedded malignant melanomas. J Immunother 1997,20:60-4. 2. Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Sakaguchi K, Apella E, et al. Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. Proc Natl Acad Sci USA 1994,91:6458-62.