Melanoma

Melan-A (Autostainer Link 48)

Clone A103

Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas and angiomyolipomas. The antibody is a useful aid for classification of melanomas and adrenocortical carcinomas. The antibody is also a useful aid for classification of angiomyolipomas.

Monoclonal Mouse Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.

Specifications


Application	Formalin HIER
Clone	A103
Code Number	IR633
Immunogen	Recombinant protein expressed in E. coli corresponding to Melan-A (1). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN₃.
Solutions	Autostainer Link 48
Species	Mouse Anti-Human
Specificity	In Western blotting of cell lysates from Melan-A mRNA positive cell lines, the antibody labels a protein doublet of 20-22 kDa, whereas no labeling is detected in Melan-A mRNA-negative cell lines (1). In immunoprecipitates of Melan-A positive cell lines SK-MEL-13 and SK-MEL-19, the antibody labels a protein doublet of 20-22 kDa corresponding to Melan-A, whereas no precipitation is detected in Melan-A mRNA-negative melanoma cell lines (1). See package insert for reference(s).

Featured References

Product Description References: 1. Fetsch PA, Cormier J, Hijazi YM. Immunocytochemical detection of MART-1 in fresh and paraffin-embedded malignant melanomas. J Immunother 1997,20:60-4.
2. Kawakami Y, Eliyahu S, Delgado CH, Robbins PF, Sakaguchi K, Apella E, et al. Identification of a human melanoma antigen recognized by tumor-infiltrating lymphocytes associated with in vivo tumor rejection. Proc Natl Acad Sci USA 1994,91:6458-62.

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