Human mast cell tryptases comprise a family of trypsin-like neutral serine proteases that are predominantly expressed in mast cells. These cells play an active role in such diverse diseases as atherosclerosis, asthma, arthritis, bile duct fibrosis, malignancy and pulmonary fibrosis. This antibody is a useful aid for classification of mast cell leukemia.
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Product Details
Features
Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Autostainer Plus
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
Specifications
Clone
AA1
Immunogen
Mast cell tryptase isolated from human lung (3).
Isotype
IgG1, kappa
Reagent Provided
Ready-to-use monoclonal mouse antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Clone: AA1 (3). Isotype: IgG1, kappa.
Solutions
Dako Autostainer/Autostainer Plus
Species
Mouse Anti-Human
Specificity
In Western blotting of a tryptase-rich extract of human lung under reducing conditions, the antibody labels a band of approximately 32.5 kDa corresponding to human mast cell tryptase (3).
In indirect ELISA the antibody reacts with purified human mast cell tryptase (3).
Antibody-binding to the mast cell tryptase does not affect enzyme activity (3).
Periodate treatment of mast cell tryptase has no effect on antibody labeling, indicating that carbohydrate epitopes are not involved in antibody recognition. Nor is antibody recognition affected by the presence of heparin (3).