The antigen used for immunization is a pool of human lambda Bence Jones proteins. Therefore, a reagent with a particularly wide specificity for lambda-chains is obtained. The specificity is directed against surface as well as hidden determinants and has been ascertained by gel precipitation techniques and immunohistochemistry. The antibody labels plasma cells and related lymphoid cells containing lambda light chains, and it is a useful aid for classification of monoclonal gammopathies.
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Product Details
Features
Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.
Specifications
Application
Formalin
HIER
Clone
Polyclonal
Code Number
GA507
Immunogen
Polyclonal light chains of type lambda isolated from a pool of normal human sera.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Solutions
Dako Omnis
Species
Rabbit Anti-Human
Specificity
The antibody reacts with free lambda chains as well as lambda chains in intact immunoglobulin molecules. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with kappa or gamma chains is seen when using 15 µL of antibody against 15 µL of kappa IgG.
Crossed immunoelectrophoresis: Only lambda-related precipitates appear when using 12.5 µL antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool ofconcentrated urines from patients with tubular proteinuria.
Cross-reaction with immunoglobulins from other species may occur. As demonstrated by immunocytochemistry, the antibody cross-reacts with immunoglobulins in dog, horse, pig and rat.