With more than 4000 literature citations, the MIB-1 antibody has now been established as an important monoclonal mouse antibody for the demonstration of the Ki-67 antigen in formalin-fixed, paraffin-embedded specimens. The Ki-67 antigen is a large nuclear protein (345, 395 kDa) preferentially expressed during all active phases of the cell cycle (G1, S, G2 and M-phases), but absent in resting cells (G0-phase). The antibody is a useful aid for classification of a variety of tumors.
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Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
HIER
Clone
MIB-1
Code Number
M7240
Immunogen
Human recombinant peptide corresponding to a 1002 bp Ki-67 cDNA fragment (6). See package insert for reference(s).
Isotype
IgG1, kappa
Reagent Provided
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, 1% bovine serum albumin, pH 7.2, and containing 15 mmol/L NaN3.
Solutions
Concentrate
Species
Mouse Anti-Human
Specificity
In Western blotting of lysates of the multiple myeloma cell line, IM-9, the MIB-1 antibody labels bands of 345 and 395 kDa, identical to the bands labeled by the original Ki-67 antibody. Furthermore, Western blotting and competitive binding experiments clearly demonstrate that MIB-1, like the original Ki-67 antibody, reacts with an epitope encoded by a 66 bp repetitive element in the Ki-67 gene. In IHC the MIB-1 and the Ki-67 antibodies provide identical staining patterns on serial tonsillar frozen sections (6). The MIB-1 antibody recognizes native Ki-67 antigen and recombinant fragments of the Ki-67 molecule (6).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Gerdes J, Becker MH, Key G, Cattoretti G. Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol 1992,168:85-6. 2. Cattoretti G, Becker MH, Key G, Duchrow M, Schlüter C, Galle J, et al. Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB 3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol 1992,168:357-63. 3. Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown [review]. J Cell Physiol 2000,182:311-22.