This antibody has been produced in a manner that ensures a particularly wide specificity for kappa-chains. The specificity is directed against surface as well as hidden determinants and has been ascertained by gel precipitation techniques and immunohistochemistry. This antibody is useful for the identification of plasma cells and related lymphoid cells containing kappa light chains, and it is a useful aid for classification of monoclonal gammopathies.
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Product Details
Features
Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.
Specifications
Application
Formalin
HIER
Clone
Polyclonal
Code Number
GA506
Immunogen
Polyclonal light chains of type kappa isolated from pooled human sera.
Reagent Provided
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
Solutions
Dako Omnis
Species
Rabbit Anti-Human
Specificity
The antibody reacts with free kappa chains as well as kappa chains in intact immunoglobulin molecules. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with lambda or gamma chains is seen when using 15 µL of concentrated antibody against 15 µL of lambda IgG.
Crossed immunoelectrophoresis: Only kappa-related precipitates appear when using 12.5 µL concentrated antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool of concentrated urines from patients with tubular proteinuria.