This antibody has been produced in a manner that ensures a particularly wide specificity for kappa-chains. The specificity is directed against surface as well as hidden determinants and has been ascertained by gel precipitation techniques and immunohistochemistry. This antibody is useful for the identification of plasma cells and related lymphoid cells containing kappa light chains, and it is a useful aid for classification of monoclonal gammopathies.
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Polyclonal immunoglobulin light chains of kappa type isolated from a pool of human sera.
Purified immunoglobulin fraction of rabbit antiserum provided in liquid form. In 0.1 mol/L NaCl, 15 mmol/L NaN3.
The antibody reacts with free kappa chains as well as kappa chains in intact immunoglobulin molecules. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins.
Specificity tests; staining with Coomassie Brilliant Blue:
Double immunodiffusion: No reaction with lambda or gamma chains is seen when using 15 µL of antibody against 15 µL of lambda IgG.
Crossed immunoelectrophoresis: Only kappa-related precipitates appear when using 12.5 µL antibody per square cm gel area against 2 µL human plasma or 2 µL of a pool ofconcentrated urines from patients with tubular proteinuria.
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.