IgG, Specific for Gamma-Chains/FITC (Conjugate) Antibody

Specifications


Application	Formalin Frozen
Clone	Polyclonal
Code Number	F0202
Immunogen	IgG isolated from a pool of normal human serum.
Reagent Provided	Polyclonal Rabbit Anti-Human IgG conjugate produced from a purified immunoglobulin fraction of rabbit antiserum. Provided in liquid form in phosphate buffer containing 15 mmol/L NaN3, pH 7.2.
Solutions	Conjugate
Species	Rabbit Anti-Human
Specificity	The antibody reacts with the γ-chains of human IgG. Traces of contaminating antibodies have been removed by solid-phase absorption. Prior to conjugation, the antibody specificity has been ascertained as follows: In crossed immunoelectrophoresis, only the IgG precipitation arch appears when using 12.5 μL unconjugated antibody, Dako Rabbit Anti-Human IgG, per cm2 gel area against 2 L human plasma. Staining: Coomassie Brilliant Blue. In indirect ELISA, no significant reaction is seen with human IgA and IgM. In double antibody sandwich ELISA, no reaction is observed with human plasma stripped of IgG. In immunofluorescence, when tested at a dilution of 1:20-1:40 on normal human formalin-fixed, paraffin-embedded tonsil, pretreated with Dako Proteinase K, the antibody gives a strong and specific staining of IgG containing plasma cells.

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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IgG, Specific for Gamma-Chains/FITC (Conjugate)

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Immunohistochemical Staining Methods - Education Guide, Sixth Edition

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