The antigen used for immunization is serum IgA. The very high specificity has been ascertained in immunohistochemistry as well as in indirect ELISA and immunoblotting. Additionally, the specificity has been tested by crossed immunoelectrophoresis using 12.5 µL antibody per square cm gel area against 2 µL human plasma.
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Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.
IgA isolated from a pool of normal human sera.
Ready-to-use polyclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L NaN3.
The antibody reacts with the alpha-chains of human IgA. Traces of contaminating antibodies have been removed by solid-phase absorption with human plasma proteins. The specificity of the antibody has been ascertained as follows:
Crossed immunoelectrophoresis: Only the IgA immunoprecipitation arch appears when using 12.5 µL concentrated antibody cm2 gel area against 2 µL human plasma. Staining: Coomassie Brilliant Blue.
ELISA: No significant reaction is seen in indirect ELISA using human IgG and IgM as coating antigens. In double antibody sandwich ELISA no significant reaction is observed with human plasma stripped of IgA.