This monoclonal rabbit antibody reacts with human estrogen receptor α (ER α). Estrogens have been found to be preferentially concentrated in the estrogen target organs of animals and in human breast cancers, and it is well documented that the mitogenic effects of estrogen are mediated by ER. Historical studies have shown that ER status is correlated with untreated outcome and with response to anti-hormonal therapy, e.g. tamoxifen (1). The antibody can used in the semi-quantitative detection of human estrogen receptor in tissue sections of human breast cancer by immunohistochemistry for assessment of estrogen receptor status.in human breast carcinomas.
This product is not available for purchase by the general public.
Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
HIER
Clone
EP1
Code Number
M3643
Immunogen
Recombinant protein of ER α amino acids 1-300.
Isotype
Rabbit IgG
Reagent Provided
Monoclonal rabbit antibody provided in liquid form as affinity-isolated antibody in 0.01 mol/L phosphate buffered saline, pH 7.2, and 0.09% sodium azide. This product contains stabilizing protein.
Solutions
Concentrate
Species
Rabbit Anti-Human
Specificity
In Western blotting of MCF7 cells lysates the antibody labels a major band of approximately 67 kDa, corresponding to the expected molecular weight of ER α. No cross-reactivity was seen with ER β.
