Prostate adenocarcinoma

ERG (Ets-Related Gene) (Dako Omnis)

Clone EP111

Ets-related gene (ERG) product is a ~41 kDa nuclear protein functioning as a DNA-binding transcriptional regulator that belongs to the erythroblast transformation-specific (ETS) family of transcription factors. In prostate cancer, ERG has most frequently been shown as a fusion protein with transmembrane protease, serine 2 (TMPRSS2), where a deletion between the TMPRSS2 and ERG genes causes the ERG gene to come under the control of the androgen-responsive promoter elements of TMPRSS2.

This antibody is a useful aid for the classification of prostate adenocarcinoma (1, 2). Furthermore, ERG may also be a useful aid for classification of prostatic intraepithelial neoplasia (PIN) and vascular tumors.

Monoclonal Rabbit Anti-Human

Features

Optimized staining performance of both high and low-expression structures
Dako Omnis and the dynamic gap staining technology provides a consistent, uniform staining with excellent morphology
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Dako Omnis
The staining performance of all FLEX RTU antibodies has been defined, tested and approved through collaboration with leading, international pathology experts.

Specifications


Application	Formalin HIER
Clone	EP111
Code Number	GA659
Immunogen	Synthetic peptide corresponding to residues in the C-terminus of human ERG.
Isotype	Rabbit IgG
Reagent Provided	Affinity-isolated ready-to-use monoclonal rabbit antibody provided in liquid form in a buffer containing stabilizing protein and 0.015 mol/L sodium azide.
Solutions	Dako Omnis
Species	Anti-Human
Specificity	In Western blotting of Jurkat, 293T and MCF-7 cell lysates the antibody labels a 55 kDa band. Immunoblot analysis on a series of expression vectors with different portions of TMPRSS2 exon 1 to ERG1 exon 2 showed that the antibody recognized all constructs except the vector without the C-terminal (Δ-term) consistent with the antibody epitope being found in the C-terminal amino acids 393-479 of the ERG protein (1). See package insert for reference(s).

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Featured References

Product Description References: 1. Park K, Tomlins SA, Mudaliar KM, Chiu YL, Esgueva R, Mehra R, et al. Antibody-based detection of ERG rearrangement-positive prostate cancer. Neoplasia 2010,12:590-8.
2. van Leenders GJ, Boormans JL, Vissers CJ, Hoogland AM, Bressers AA, Furusato B, et al. Antibody EPR3864 is specific for ERG genomic fusions in prostate cancer: implications for pathological practice. Mod Pathol 2011,24:1128-38.

ERG (Ets-Related Gene) (Dako Omnis)

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