Epstein-Barr Virus, LMP Antibody (Autostainer Link 48)

Features

Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.

Specifications


Application	Formalin HIER
Clone	CS.1-4
Code Number	IR753
Immunogen	Recombinant fusion protein containing sequences of bacterial beta-galactosidase and the carboxyl half of EBV-encoded LMP (7). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Ready-to-use mixture of four monoclonal mouse antibodies provided in liquid form as tissue culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2 containing 0.015 mol/L NaN₃.
Solutions	Autostainer Link 48
Species	Mouse Anti-
Specificity	In Western blotting with lysates of EBV-transformed lymphoblastoid cell lines, the antibody labels a major band of 57-66 kDa depending on the virus isolate, sometimes accompanied by a minor band of 50-55 kDa corresponding to full length and truncated form of LMP. The antibody recognizes 20 geographically distinct EBV isolates (7). The antibody cross-reacts with a 43-44 kDa doublet of uncharacterized normal cell proteins in cellular extracts including that from EBV-negative control cell lines (7). The antibody labels a normal EBV-positive lymphoblastoid cell line established by infection of normal resting B-cells with B95.8 virus, whereas the BL2 cell line, established from a rare EBV-negative sporadic BL-patient, as well as other unrelated cell isolates, including tonsillar B and T lymphocytes, fetal liver and thymus, cultured fetal fibroblasts, human leukaemic cell lines HSB2 and K562 and 7 EBV-negative B-cell lines show no labeling, except the cellular doublet proteins (7). See package insert for reference(s).

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Literature

Catalogs

Atlas of Controls

The Atlas of Controls illustrates the staining performance of each antibody in the FLEX Ready-to-Use system.

Catalogs
English
26 Mar 2024
37.78 MB
PDF

Technical Overviews

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Featured References

Product Description References: 1. Pallesen G, Hamilton-Dutoit SJ, Rowe M, Young LS. Expression of Epstein-Barr virus latent gene products in tumour cells of Hodgkin's disease. Lancet 1991,337:320-2.
2. Vera-Sempere FJ, Burgos JS, Botella MS, Cordoba J, Gobernado M. Immunohistochemical expression of Epstein-Barr virus-encoded latent membrane protein (LMP-1) in paraffin sections of EBV-associated nasopharyngeal carcinoma in Spanish patients. Oral Oncol, Eur J Cancer 1996,32B:163-8.

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Epstein-Barr Virus, LMP (Autostainer Link 48)

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Atlas of Controls

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