Epithelial Antigen Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Frozen Formalin HIER
Clone	Ber-EP4
Code Number	M0804
Immunogen	MCF-7 cells (human breast carcinoma cell line) (4). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN₃.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	Analysis of immunocomplexes between the antibody and lysate of ¹²⁵I surface-labeled MCF-7 cells in SDS-PAGE under reducing conditions shows that the antibody labels two polypeptides of 34 kDa and 39 kDa, respectively, corresponding to epithelial antigen. Under non-reducing conditions, the polypeptides appear as 39 kDa and 41 kDa, while deglycosylation reduces the size to 31 kDa, and 36 kDa. In immunoprecipitation experiments, the Ber-EP4 antibody blocks the reaction of the HEA125 antibody with MCF-7 cell lysate and vice versa, showing that the two antibodies react with the same antigen. The two antibodies also produce identical staining results in cells and tissues (4). Of 37 cell lines tested, the antibody homogeneously labels all (10/10) carcinoma cell lines, whereas all non-epithelial cell lines (26/27) are not labeled except for the erythromyeloid cell line K562 (4). See package insert for reference(s).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Epithelial Antigen (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

View supported Instructions For Use documents

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