Reacts with CMV immediate early antigen and early antigen. The antibody shows no cross-reaction with other herpesviruses or with adenovirus. In CMV-infected cells, the antibody gives a nuclear staining pattern early during the infection; at a later stage, a diffuse nuclear and apparent cytoplasmic staining is observed. The antibody is particularly well-suited for the detection of CMV in infected human embryonic fibroblasts.
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Optimized staining performance of both high and low-expression structures
Crisp and clear staining with no background
Optimal laboratory efficiency with ready-to-use antibodies on Autostainer Link 48
The staining performance of all antibodies has been defined, tested and approved through collaboration with leading international pathologists. Check the Atlas of Stains - a guide to diagnostic accuracy.
CCH2 + DDG9
Crude cell lysates from cytomegalovirus strain Ad169-infected human embryonic fibroblasts (3). See package insert for reference(s).
IgG2a, kappa and IgG1, kappa
Ready-to-use mixture of two monoclonal mouse antibodies provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, 0.015 mol/L NaN3, pH 7.2.
Autostainer Link 48
Antibody CCH2 reacts with an early nuclear protein identical with the non-structural DNA-binding protein p52 (7). More than 200 human CMV isolates have been successfully typed by this antibody which, therefore, seems to recognize an epitope which is highly conserved among HCMV strains (3, 4). Antibody DDG9 reacts with an immediate early nuclear protein of about 76 kDa (3). For both antibodies, the reactivity persists also at later stages during HCMV infection where localization is less distinctly nuclear and appears to be in the cytoplasm. Laser confocal microscopy, however, shows that the reaction is limited to the nuclear membrane (3). The antibodies do not cross-react with adenovirus, herpes simplex virus, and varicella zoster virus (3). See package insert for reference(s).
1. Niedobitek G, Finn T, Herbst H, et al. Detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using the new monoclonal antibody CCH2: a comparison of methods. J Clin Pathol 1988,41:1005-9. 2. Wirgart BZ, Landqvist M, Hökeberg I, et al. Early detection of cytomegalovirus in cell culture by a new monoclonal antibody, CCH2. J Virol Methods 1990,27:211-20.