Cytokeratins 8 and 18 (CK8/18) make up one of the low molecular weight cytokeratins (LMW-CK). CK8/18 is expressed in simple, non-stratified epithelia, basal and superficial cells of transitional epithelium, the luminal/secretory cells of complex epithelia, mesothelium, and may be present in some types of mesenchymal cells. Multiple cytokeratins family members may be expressed in a given cell and are characteristic of the cell type and differentiation state. Nearly all carcinomas of epithelial origin and mesotheliomas express CK8/18, and CK8/18 expression patterns aid in the classification of tumors of unknown origin and poorly differentiated carcinomas. Antibodies to CK8/18 may be useful for classification of tumors of epithelial origin (1).
This product is a cocktail of two monoclonal rabbit antibodies.
This product is not available for purchase by the general public.
Product Details
Features
Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years experience ensuring quality and innovation in antibodies and reagents
Specifications
Application
Formalin
HIER
Clone
EP17/EP30
Code Number
M3652
Immunogen
CK8: A synthetic peptide corresponding to residues in the C-terminal part of human cytokeratin 8 protein. CK18: Human cytokeratin 18 protein isolated from HT-29 cells.
Isotype
Rabbit IgG
Reagent Provided
A mixture of two monoclonal rabbit antibodies provided in liquid form as cell culture supernatant in 0.05 mol/L Tris-HCl, 0.015 mol/L sodium azide, pH 7.2. This product contains stabilizing protein.
Solutions
Concentrate
Species
Anti-Human
Specificity
CK8/18 is a cocktail of two monoclonal antibodies. In Western blotting of A431 cell lysates, anti-CK8, clone EP17 recognizes a major band of 52 kDa corresponding to the expected molecular weight of CK8; and anti-CK18, clone EP30 recognizes a major band of 45 kDa corresponding to the expected molecular weight of CK18.
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.