Reacts with the 65-67, 64, 59, 58, 56.5, 56, 54, 52, 50, 48 and 40 kDa cytokeratins. The antibody labels keratinized and corneal epidermis, stratified squamous epithelia of internal organs, stratified epithelia, hyperproliferative keratinocytes, and simple epithelia. The antibody is useful aid for classification of tumors of epithelial origin as well as undifferentiated carcinomas.
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Human epidermal callus (1). See package insert for reference(s).
Monoclonal mouse antibody provided in liquid form as tissue culture supernatant in 0.05 mol/L Tris-HCl, pH 7.2 and 0.015 mol/L sodium azide. This product contains stabilizing protein.
AE1/AE3 is a cocktail of two monoclonal antibodies that were obtained by immunizing mice with human callus keratins (2). AE1/AE3 has been shown to identify the majority of human cytokeratins and thus may be used as a tool for the positive IHC identification of cells of simple and stratified epithelial origin (1,2,4). Antibody AE1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with Moll's designation (4) 10, 13, 14, 15 16 and 19 (MWs of 56.5, 54', 50, 50', 48 and 40 kDa, respectively) but not on Nos. 12, 17 and 18 (55, 47 and 45 kDa) (4). Antibody AE3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including Nos. 1 and 2, 3, 4, 5, 6, 7 and 8 (MWs of 65, 67, 64, 59, 58, 56, 54 and 52 kDa, respectively) (5). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.