Collagen IV Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Frozen Formalin HIER
Clone	CIV 22
Code Number	M0785
Immunogen	Purified pepsin fragments of type IV collagen isolated from human kidney (2). See package insert for reference(s).
Isotype	IgG1, kappa
Reagent Provided	Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 50 mmol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN₃.
Solutions	Concentrate
Species	Mouse Anti-Human
Specificity	The specificity of the antibody has been assessed in radioimmunoassay (RIA) where it recognizes an epitope present in the human type IV collagen in native conformation, whereas it does not recognize the reduced and alkylated protein in the denatured state. Staining of immunoblots with the antibody was negative, further indicating that the antibody recognizes a conformational epitope on type IV collagen (2). In immunoblotting or RIA, no cross-reactivity of the antibody with isolated human collagen types I, II, III and V could be detected (2). See package insert for reference(s).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Collagen IV (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

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