Reacts with a 130 kDa glycoprotein, also designated platelet endothelial cell adhesion molecule-1 (PECAM-1). The antibody strongly labels endothelial cells and is a useful aid for classification of neoplasms arising from endothelial cells.
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Cell membrane preparation from the spleen of a patient with hairy cell leukemia (1). See package insert for reference(s).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
The antibody was clustered as anti-CD31 at the Fifth International Workshop and Conference on Human Leucocyte Differentiation Antigens (4). The epitope recognized was found to be within the extracellular domain 1 (3). In Western blotting of membrane preparations from a spleen rich in the antigen or from normal platelets, the antibody labels bands of respectively 100 kDa and 130 kDa, the latter corresponding to classic CD31. The smaller band of 100 kDa observed with the splenic preparation may be due to proteolytic breakdown or to variations in glycosylation (1). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
30 Nov 2021
Product Description References
1. Parums DV, Cordell JL, Micklem K, Heryet AR, Gatter KC, Mason DY. JC70: a monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol 1990,43:572-7. 2. Kuzu I, Bicknell R, Harris AL, Jones M, Gatter KC, Mason DY. Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol 1992,45:143-8. 3. Ohsawa M, Naka N, Tomita Y, Kawamori D, Kanno H, Aozasa K. Use of immunohistochemical procedures in diagnosing angiosarcoma. Evaluation of 98 cases. Cancer 1995,75:2867-74.