Recognizes a formalin-resistant epitope in both the 80 kDa NPM-ALK chimeric and the 200 kDa normal human ALK proteins (1). Normal ALK protein expression is restricted to the central nervous system. The hybrid gene, NPM-ALK, created by the t(2;5)(p23;q35) chromosomal translocation encodes part of the nucleolar phosphoprotein, nucleophosmin (NPM), joined to the cytoplasmic portion of the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase (2). This antibody is a useful aid for classification of anaplastic large cell lymphoma (ALCL).
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DHFR-ALK recombinant protein comprising mouse dihydrofolate reductase (DHFR) and amino acids 1359-1460 of the full length human ALK protein, corresponding to amino acids 419-520 of the chimeric NPM-ALK protein (1). See package insert for reference(s).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
The antibody was clustered as anti-CD246 at the Seventh International Workshop and Conference on Human Leucocyte Differentiation Antigens held in Harrogate in 2000 (3). In Western blotting of lysate of the t(2;5)+ SU-DHL-1 cell line, the antibody labels a band of 80 kDa corresponding to the NPM-ALK protein. The antibody also recognizes a band of 200 kDa corresponding to the ALK protein in the human rhabdomyosarcoma cell line Rh30, that expresses full length ALK protein, and in the 293T cell line transfected with cDNA encoding full length ALK protein. No reactivity was observed when the 293T cell line was transfected with the vector alone (1). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Pulford K, Lamant L, Morris SW, Butler LH, Wood KM, Stroud D, et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood 1997,89:1394-404. 2. Morris SW, Kirstein MN, Valentine MB, Dittmer KG, Shapiro DN, Saltman DL, et al. Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin's lymphoma (published erratum appears in Science 1995,267:316-7). Science 1994,263:1281-4.