CD117, c-kit Antibody (Concentrate)

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	Polyclonal
Code Number	A4502
Immunogen	Peptide corresponding to amino acids 963 to 976 at the cytoplasmic C-terminal part of c-kit (1,5). See package insert for reference(s).
Reagent Provided	Affinity-isolated rabbit antibody purified through antigen-bound, activated thiol AvidGel F affinity chromatography and provided in liquid form, in 0.05 mol/L Tris-HCl, 0.1 mol/L NaCl, 0.015 mol/L NaN₃, pH 7.2, 1% bovine serum albumin.
Solutions	Concentrate
Species	Rabbit Anti-Human
Specificity	In Western blotting of an extract of the small cell lung carcinoma cell line SY, that over-expresses c-kit mRNA, the antibody labels a band of 145 kDa corresponding to the c-kit protein. The labeled band is rather broad, from 120 to 155 kDa. An additional band of 100 kDa is also labeled (1). In another study applying a different antibody to c-kit, a 100 kDa protein was, likewise, labeled. This labeling was abolished when the antibody was incubated with the synthetic c-kit peptide antigen used for immunization (6). In Western blotting, the antibody did not react with an extract of the adenocarcinoma cell line HS, that is without c-kit gene expression (1). See package insert for reference(s).

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Literature

Technical Overviews

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.

Technical Overviews
English
30 Nov 2021
27.05 MB
PDF

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Featured References

Product Description References: 1. Tsuura Y, Hiraki H, Watanabe K, Igarashi S, Shimamura K, Fukuda T, et al. Preferential localization of c-kit product in tissue mast cells, basal cells of skin, epithelial cells of breast, small cell lung carcinoma and seminoma/dysgerminoma in human: immunohistochemical study on formalin-fixed, paraffin-embedded tissues. Virchows Arch 1994,424:135-41.
2. van Oosterom AT, Judson I, Verweij J, Stroobants S, di Paola ED, Dimitrijevic S, et al. Safety and efficacy of imatinib (STI571) in metastatic gastrointestinal stromal tumours: a phase I study. Lancet 2001,358:1421-3.
3. Hornick JL, Fletcher CDM. Immunohistochemical staining for KIT (CD117) in soft tissue sarcomas is very limited in distribution. Am J Clin Pathol 2002,117:188-93
4. Smithey BE, Pappo AS, Hill DA. C-kit expression in pediatric solid tumors: a comparative immunohistochemical study. Am J Surg Pathol 2002,26:486-92.

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CD117, c-kit (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

View supported Instructions For Use documents

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