The BCL6 gene encodes a 706 amino acid nuclear protein of the Kruppel-type zinc finger protein. It is rearranged in about 30% of diffuse large B-cell lymphomas, and is expressed predominantly in normal germinal centre B cells and related lymphomas . The antibody is a useful aid for classification of follicular lymphomas, diffuse large B-cell lymphomas, Burkitt's lymphomas, and nodular, lymphocyte-predominance Hodgkin's lymphoma. The BCL6 antibody, together with BCL2 antibody, is also a useful aid in classification of mantle cell lymphomas, and nodular, lymphocyte-predominance Hodgkin's lymphoma. BCL6 protein is not expressed in B-CLL, hairy cell leukemia, mantle cell and marginal-zone derived lymphomas (1, 2).
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Recombinant BCL6 protein corresponding to amino acids 3-484 of the human BCL6 protein (1). See package insert for reference(s).
Monoclonal mouse antibody provided in liquid form as cell culture supernatant dialysed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 15 mmol/L NaN3.
In Western blotting of total lysates of Bjab cells and BCL6 transfected EB3 cells, or control transfected EB3 cells, the antibody did not label any bands, suggesting lack of reactivity with denatured BCL6 protein (1). SDS-PAGE analysis of immunoprecipitates formed between total lysates of Bjab cells (BCL6+) and BCL6 transfected EB3 cells and the antibody, shows reaction with a ~95 kDa fragment corresponding to BCL6. No reaction is observed with Rd cells (BCL6-) and EB3 cells transfected with a control plasmid (1). See package insert for reference(s).
The focus of the sixth edition of the IHC Guidebook is to provide a comprehensive immunohistochemistry (IHC) resource for lab managers, lab technicians, learning pathologists, and students from around the world.
1. Flenghi L, Bigerna B, Fizzotti M, Venturi S, Pasqualucci L, Pileri S, et al. Monoclonal antibodies PG-B6a and PG-B6p recognize, respectively, a highly conserved and a formol-resistant epitope on the human BCL-6 protein amino-terminal region. Am J Pathol 1996,148:1543-55. 2. Falini B, Bigerna B, Pasqualucci L, Fizzotti M, Martelli MF, Pileri S, et al. Distinctive expression pattern of the BCL-6 protein in nodular lymphocyte predominance Hodgkin's disease. Blood 1996,87:465-71.