Prostate adenocarcinoma

AMACR (Concentrate)

Clone 13H4

Recognizes a 382-amino-acid protein, alpha-methylacyl-CoA racemase (AMACR), that was identified by cDNA library subtraction in conjunction with high throughput microarray screening of prostate adenocarcinomas. AMACR, also known as P504S, is an enzyme that is involved in bile acid biosynthesis and β-oxidation of branched-chain fatty acids. Results aid in the classification of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma (1). AMACR is present at low or undetectable levels in glandular epithelial cells of normal and benign hyperplastic prostates.

Monoclonal Rabbit Anti-Human

Features

Excellent specificity
High lot to lot consistency
Optimized for immunohistochemistry (IHC) with validated protocols
Certified manufacturing facilities guarantee full quality control
More than 50 years’ experience ensuring quality and innovation in antibodies and reagents

Specifications


Application	Formalin HIER
Clone	13H4
Code Number	M3616
Immunogen	Full length recombinant AMACR (9). See package insert for reference(s).
Isotype	Rabbit IgG
Reagent Provided	Monoclonal rabbit antibody provided in liquid form as cell culture supernatant (containing fetal bovine serum) dialyzed against 0.05 mol/L Tris-HCl, pH 7.2, and containing 0.015 mol/L sodium azide. This product contains stabilizing protein.
Solutions	Concentrate
Species	Rabbit Anti-Human
Specificity	The specificity of monoclonal rabbit anti-AMACR was evaluated by immunocytochemistry and Western blot analysis. Anti-AMACR positively bound formalin-fixed HEK293 cells which overexpressed AMACR, but was unreactive with cells transfected with an empty plasmid. In Western blots of lysates from primary prostate carcinoma samples, monoclonal rabbit anti-AMACR identified a 54 kDa protein corresponding to the expected molecular weight of AMACR (2). See package insert for reference(s).

Return to top

Featured References

Product Description References: 1. Luo J, Zha S, Gage WR, Dunn TA, Hicks JL, Bennett CJ, et al. Alpha-methylacyl-CoA racemase: a new molecular marker for prostate cancer. Cancer Res 2002,62:220-6.

AMACR (Concentrate)

Product Details

Immunohistochemical Staining Methods - Education Guide, Sixth Edition

View supported Instructions For Use documents

1. Luo J, Zha S, Gage WR, Dunn TA, Hicks JL, Bennett CJ, et al. Alpha-methylacyl-CoA racemase: a new molecular marker for prostate cancer. Cancer Res 2002,62:220-6.

Customers Also Viewed These Products