Tris-Buffered NaCl Solution with Tween 20, pH 7.6

Makes 5 L of working buffer, 0.05 mol/L Tris/HCl, 0.30 mol/L NaCl, 0.1% Tween 20, pH 7.6. Contains a preservative. The relatively high salt concentration makes this buffer particularly well-suited for immunohistochemical staining methods that require very thorough washing and for in situ hybridization procedures.