This product is a modified citrate buffer, pH 6.1 provided as 10x concentrate. Using this TRS for heat-induced target retrieval significantly improves the staining results obtained with many previously unreactive antigens/antibodies in formalin-fixed, paraffin-embedded tissue sections compared to using a standard 0.01 mol/L citrate buffer, pH 6.0.
It may also be useful for in situ hybridization procedures.
Prior to use this product must be diluted 1:10 with deionized or distilled water (one part TRS plus nine parts water) to make a working solution. The final pH should be between 6.00 and 6.20 (optimally 6.10).