The PL aquagel-OH size exclusion chromatography (SEC) column offers efficient and reliable separation of water-soluble polymers and large molecules, such as PEG, polysorbate, starch, dextran, and acrylamide. PL aquagel-OH columns meet USP L25, tolerate high buffer concentrations, pH 2 to 10, temperatures up to 90 °C, and organic solvents (up to 50% methanol).