GPC/SEC Columns Selector Tools
Agilent offers a series of tools to help you navigate to the best GPC/SEC column choice. Guidance can be by:
- Mobile Phase
- Application
- Manufacturer’s part number cross-reference
- USP designation
- EP monograph
Pullulan Standards
Pullulan standards are commonly used for aqueous GPC/SEC column calibration. These individual polysaccharide reference standards are available with molar masses ranging from 342 to 1,000,000 Da. Pullulan calibration kits and ReadyCal kits are also available, with molecular weights up to 1,000,000 Da.
Product Details
Features
- Aqueous polysaccharide standards for GPC/SEC column and system calibration
- Individual molecular weight polymer standards available from 342 to 1,000,000 Da
- Molecular weights selected to provide equidistant calibration points for greater accuracy and linear calibration
- Comprehensively characterized by GPC/SEC, light scattering, and NMR
- Quality certificate is available for download and provides characterization information, including testing parameters and at least the molecular weight values (Mn, Mw, Mp, PDI)
- Membrane characterization standards available with broad molar mass range
- Traditional calibration kits and ReadyCal kits also available
How It Works
How To Analyze Polymers, Biopolymers, and Proteins with GPC/SEC
GPC/SEC measures molecular weight distribution and averages for natural and synthetic polymers. This eBook discusses how to analyze polymers, biopolymers, and proteins with GPC/SEC. Topics include:
- Quantify and get more than molar mass averages
- Protein analysis with SEC
- Calibration using broad standards
- Analyzing high molar mass samples
- Branching analysis
- GPC/SEC for filter analysis
Greener Gel Permeation Chromatography (GPC) and Size Exclusion Chromatography (SEC)
This white paper discusses less hazardous solvent alternatives from renewable resources that can be successfully applied for size separation of macromolecules. Options to establish solutions that align with the 12 principles of green chemistry are also presented. Three of the 12 green chemistry principles are particularly applicable in liquid chromatography:
- Waste Prevention
- Safer Solvents and Auxiliaries
- Use of Renewable Feedstocks
GPC/SEC Calibration Kits
Kits of well-characterized polymer standards for aqueous or organic GPC/SEC column calibration
Category: GPC/SEC Standards
ReadyCal Kits
Kits for quick, reproducible 8 to 12 point calibration curves using convenient, preweighed standards
Category: GPC/SEC Standards