Seahorse XF Mito Fuel Flex Test Kit
RUO
The Agilent Seahorse Mito Fuel Flex Test kit provides an analytical method for measuring the basal state of mitochondrial fuel oxidation in live cells. This assay kit utilizes a set of inhibitors to reveal the cells' ability to switch oxidative pathways to meet basal energetic demands. It provides information on the relative contributions of glucose, glutamine, and long-chain fatty acid oxidation to basal respiration.
Each Mito Fuel Flex Test kit contains the materials sufficient for six full-plate tests.
Each Mito Fuel Flex Test kit contains the materials sufficient for six full-plate tests.
For Research Use Only. Not for use in diagnostic procedures.
- XF Assay Kits, Reagents & Cell Assay Media
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Product Details
Features
- Uses specific inhibitors in different combinations to measure the dependency, capacity, and flexibility of cells to oxidize three major mitochondrial fuels: glucose (pyruvate), glutamine (glutamate), and long-chain fatty acids in basal energetic state
- Compounds are reconstituted directly in the assay medium, eliminating the need for DMSO or other solvents
- Each lot is rigorously tested and validated with functional assays to deliver consistent and robust results, eliminating the costs associated with failed experiments
- The Agilent Seahorse Mito Fuel Flex Test report generator simplifies data processing and transforms raw data into organized, shareable summary reports
How It Works
Literature
- Flyers
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Agilent Seahorse XF Assay Kits and Reagents
This documents contains high level description for all Agilent seahorse XF kits and reagents, include key features and benefits
- Flyers
- English
- 17 Mar 2020
- 932.16 KB
XF Mito Fuel Flex Test Kit
Measure mitochondrial fuel (glucose, glutamine, fatty acid) usage in live cells.
- Flyers
- English
- 19 Mar 2018
- 2.12 MB
- Posters
Support
- FAQs
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Why is a 1-hour non-CO2 incubation necessary for the cell plate?
This 1-hour incubation is necessary for de-gassing the plate, allowing for CO2 diffusion from the cells, medium, and plate.
How are the metabolic parameters calculated in the Seahorse XF Report Generators?
The values used for each Seahorse XF Report Generator calculation are outlined in the specific Seahorse XF Report Generator assay user guide.
- Supplemental Information
- User Manuals
Tools
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Cell Analysis Product Selection Guide
Select the best assays and hardware technology for your research area.
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