Seahorse XF Cell Mito Stress Test Kit
Each kit contains the materials sufficient for six full plate tests and is available in two packaging sizes. The XF kit is for use with Agilent Seahorse XF/XFe96 and XF/XFe24 Analyzers and the XFp kit is for use with Agilent Seahorse XFp Analyzers.
- XF Assay Kits, Reagents & Cell Assay Media
Product Details
- The assay measures basal respiration, ATP-linked respiration, H+ (proton) leak, maximal respiration, spare respiratory capacity, and non-mitochondrial respiration using modulators of cellular respiration
- Compounds are reconstituted directly in the assay medium, eliminating the need for DMSO or other solvents
- Each lot is rigorously tested and validated with functional assays to deliver consistent and robust results, eliminating the costs associated with failed experiments
- Upload your assay result file to your Agilent Seahorse Analytics account to calculate and report parameters of the XF Cell Mito Stress Test, share with collaborators, or export data to Microsoft Excel or GraphPad Prism for other graphing or analysis needs
- Application Notes
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Gaining Insights into Disease Biology for Target Identification and Validation
This Application Note shows how Seahorse XF technology can be used to determine specific metabolic states associated with cellular dysfunction and disease
- Application Notes
- English
- 12 Nov 2019
- 572.72 KB
Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets
An overview of how the Agilent Seahorse XF Cell Mito Stress Test and XF PMP workflows can be applied to mitochondrial and metabolic target identification studies.
- Application Notes
- English
- 10 Jun 2019
- 389.41 KB
Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia
Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment
- Application Notes
- English
- 03 Jan 2017
- 744.28 KB
Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer
Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer
- Application Notes
- English
- 22 Dec 2016
- 1.07 MB
Assessing therapies for traumatic brain or spinal cord injuries
XF technology provides insight into treatment interventions for mitochondrial dysfunction
- Application Notes
- English
- 22 Dec 2016
- 679.93 KB
Assessing the impact of autophagy on cellular metabolism
Real-time, functional metabolic assays connect energy pathways and autophagy
- Application Notes
- English
- 22 Dec 2016
- 1.06 MB
- Flyers
- Posters
- Selection Guides
- Technical Overviews
- FAQs
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How are the metabolic parameters calculated in the Seahorse XF Report Generators?
The values used for each Seahorse XF Report Generator calculation are outlined in the specific Seahorse XF Report Generator assay user guide.
Why is a 1-hour non-CO2 incubation necessary for the cell plate?
This 1-hour incubation is necessary for de-gassing the plate, allowing for CO2 diffusion from the cells, medium, and plate.
- Supplemental Information
- User Manuals
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Cell Analysis Product Selection Guide
Select the best assays and hardware technology for your research area.