Seahorse XFe96 Analyzer
OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
- XF Analyzers
Product Details
- 96-well plate format accommodates many conditions in a single run, for flexible assay design, dose-response studies, and screening
- Reports real-time metabolic rates in minutes, without sample extraction or labelling
- Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time
- High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
- Precision-controlled heating tray, maintains 16–42°C temperatures (12–20°C above ambient conditions), for compatibility with a variety of sample types
- Quickly determine dependency of cellular energy production on mitochondrial substrates
- Generate a metabolic phenotype within one hour for fast data turnaround
- Analyze 3D samples such as spheroids and islets
- Wave software allows easy assay protocol creation and data analysis on your desktop PC and export to common spreadsheet and graphing programs
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- Application Notes
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Gaining Insights into Disease Biology for Target Identification and Validation
This Application Note shows how Seahorse XF technology can be used to determine specific metabolic states associated with cellular dysfunction and disease
- Application Notes
- English
- 12 Nov 2019
- 572.72 KB
Maximize Efficiency & Performance of Seahorse XF Assays with the Bravo Liquid Handler
Bravo is used to automate three liquid handling steps for Seahorse XFe96 assay preparation: washing cells, preparing injection solutions, sensor cartridge loading.
- Application Notes
- English
- 12 Nov 2019
- 1.75 MB
Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets
An overview of how the Agilent Seahorse XF Cell Mito Stress Test and XF PMP workflows can be applied to mitochondrial and metabolic target identification studies.
- Application Notes
- English
- 10 Jun 2019
- 389.41 KB
XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System
This application note highlights how the XF Imaging and Normalization System provides an efficient and reliable method of normalization with a seamless and rapid workflow for XF Analyzer users.
- Application Notes
- English
- 01 Nov 2018
- 719.16 KB
Evaluating Changes in Cell Metabolism in Neuroimmune and Neuropsychiatric Disorders
Application Brief- summarizes scientific Agilent-Seahorse XF research focused on neurodegeneration, immunology, and infectious disease.
- Application Notes
- English
- 17 May 2017
- 689.46 KB
Normalization of Agilent Seahorse XF Data Data by In-situ Cell Counting Using a Biotek Cytation 5
Application Note - Normalization of Agilent Seahorse XF data by in-situ cell counting using a BioTek Cytation 5
- Application Notes
- English
- 24 Mar 2017
- 931.07 KB
Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia
Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment
- Application Notes
- English
- 03 Jan 2017
- 744.28 KB
Measuring functional metabolism of brite adipocytes
Mitochondrial function correlates to brown adipocyte function
- Application Notes
- English
- 08 Mar 2017
- 1.04 MB
Measuring bioenergetic profiles of human adipocytes
Measuring white adipose tissue (WAT) mitochondrial function reveals impaired fatty acid stimulated uncoupling from obese individuals.
- Application Notes
- English
- 07 Mar 2017
- 725.56 KB
- Brochures
- Case Studies
- Flyers
- Ordering Guides
- Posters
- Selection Guides
- Technical Overviews
- FAQs
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How do I use the injection ports?
Seahorse has developed the Seahorse XF Cell Stress Test kit consisting of pre-calibrated, controlled reagents to simplify the process of teasing out parameters of mitochondrial respiration and glycolysis. However, any soluble compounds may be added through the injection ports of the cartridge. The Seahorse scientific support team is available to assist with experimental design and data analysis for your application.
How does Seahorse XF Technology work?
Seahorse XF technology combines solid-state sensors, electro-optical detection and proprietary consumables to enable the real-time measurement of cell metabolism in minutes. The sensors in XF cartridges measure the flux of oxygen and protons in the transient microchambers created by our specially designed tissue culture microplates in real time. The software translates the flux to the rates of oxygen consumption and extracellular acidification. For additional details, visit our How It Works webpage.
How do I exclude "outlier" wells from my data analysis?
How do I exclude "outlier" wells from my data analysis?
- Instruction Sheets
- Quick Reference Guides
- Site Preparation Checklists
- Software Utilities
- Supplemental Information
- User Manuals
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Cell Analysis Product Selection Guide
Select the best assays and hardware technology for your research area.
Videos
Probe the Metabolic Depths of Tumor Spheroids Using the XFe96 Analyzer
In this webinar we will discuss the use of the new XFe96 Spheroid Microplate, a 96-well microplate with unique geometry that enables functional, metabolic measurements of individual spheroids.
Seahorse Metabolic Analyzers Enable Rapid, Quantitative Measurement of Cellular Bioenergetics
Seahorse assays enable quantification of cellular bioenergetics by measuring the two energy yielding pathways, mitochondrial respiration and glycolysis, in a sensitive and convenient microplate format.
BioTek is now a part of Agilent
A global leader in the design and manufacture of innovative life science instrumentation, including cell imaging systems, microplate readers, washers, dispensers, automated incubators, and stackers, broadens our cell analysis solution offerings.
- 12 Mar 2020