Seahorse XFe96 Analyzer
RUO
Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format.
OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.
For Research Use Only. Not for use in diagnostic procedures.
Product Details
Features
- 96-well plate format accommodates many conditions in a single run, for flexible assay design, dose-response studies, and screening
- Reports real-time metabolic rates in minutes, without sample extraction or labelling
- Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time
- High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate
- Precision-controlled heating tray, maintains 16–42°C temperatures (12–20°C above ambient conditions), for compatibility with a variety of sample types
- Quickly determine dependency of cellular energy production on mitochondrial substrates
- Generate a metabolic phenotype within one hour for fast data turnaround
- Analyze 3D samples such as spheroids and islets
- Wave software allows easy assay protocol creation and data analysis on your desktop PC and export to common spreadsheet and graphing programs
How It Works
Applications
Featured References
- Publications
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- Gibellini, L., et al. Altered bioenergetics and mitochondrial dysfunction of monocytes in patients with COVID-19 pneumonia. EMBO Mol Med. 2020. 12 (12): e13001. Learn More
- Li, Y., et al. MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme. Nat Commun. 2020. 11 (1): 4837. Learn More
- Brookens, S. K., et al. AMPKalpha1 in B Cells Dampens Primary Antibody Responses yet Promotes Mitochondrial Homeostasis and Persistence of B Cell Memory. J Immunol. 2020. 205 (11): 3011–3022. Learn More
- Koss, B., et al. Epigenetic Control of Cdkn2a.Arf Protects Tumor-Infiltrating Lymphocytes from Metabolic Exhaustion. Cancer Res. 2020. 80 (21): 4707–4719. Learn More
- Polak, A., et al. SYK inhibition targets acute myeloid leukemia stem cells by blocking their oxidative metabolism. Cell Death Dis 11, 956 (2020). Learn More
- Ren, Z. et al. Insulin Promotes Mitochondrial Respiration and Survival through PI3K/AKT/GSK3 Pathway in Human Embryonic Stem Cells. Stem Cell Reports 2020, 15 (6), 1362-1376. Learn More
- Metabolic reprogramming of osteoclasts represents a therapeutic target during the treatment of osteoporosis. Scientific Reports 2020, 10 (1), 21020. Learn More
- Calderon A, et al. Identification of Mubritinib (TAK 165) as an inhibitor of KSHV driven primary effusion lymphoma via disruption of mitochondrial OXPHOS metabolism. Oncotarget. 2020 Nov 17;11(46):4224–4242. Learn More
- Dowling CM, et al. Multiple screening approaches reveal HDAC6 as a novel regulator of glycolytic metabolism in triple-negative breast cancer. Sci Adv. 2021;7(3):eabc4897. Learn More