Seahorse XFe24 Analyzer
RUO
Agilent Seahorse XFe24 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 24-well plate format.
OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate, and together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate, and together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.
For Research Use Only. Not for use in diagnostic procedures.
Product Details
Features
- 24-well microplate format, provides the capacity for larger and/or more metabolically active samples
- Reports real-time metabolic rates in minutes, without sample extraction or labeling
- Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time
- High sensitivity to analyze as few as 10,000 cells per well in the custom 24-well plate
- Precision-controlled heating tray, maintains temperatures at 16–42°C (12–20°C above ambient conditions), for compatibility with a variety of samples
- Analyze 3D samples such as zebrafish and islets
- Quickly determine dependency of cellular energy production on mitochondrial substrates
- Wave software allows easy creation of assay protocols and data analysis on your desktop PC and export to common spreadsheet and graphing programs
How It Works
Applications
Featured References
- Publications
-
- Frenkel, J. D. H. et al. Metformin enhances protection in guinea pigs chronically infected with Mycobacterium tuberculosis. Sci Rep. 2020. 10 (1): 16257. Learn More
- Demare, S., et al. Metformin as a potential therapeutic for neurological disease: mobilizing AMPK to repair the nervous system. Expert Rev Neurother. 2021. 21 (1): 45–63. Learn More
- Masoud, R., et al. Targeting Mitochondrial Complex I Overcomes Chemoresistance in High OXPHOS Pancreatic Cancer. Cell Rep Med. 2020. 1 (8): 100143. Learn More
- Yang, Y., et al. Mitochondrial UQCC3 Modulates Hypoxia Adaptation by Orchestrating OXPHOS and Glycolysis in Hepatocellular Carcinoma. Cell Reports 2020, 33 (5), 108340. Learn More
- Levoux J, et al. Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Learn More
