MitoXpress Intra Intracellular Oxygen Assay
The Agilent MitoXpress Intra intracellular oxygen assay is a unique and powerful tool for the quantification of real-time intracellular oxygenation. Specifically, the assay measures intracellular and pericellular oxygen concentration using a nano sensor probe. Oxygen concentration is a key parameter across many fields of research, including hypoxia, oxidative stress, and cancer metabolism.
- Plate Reader Metabolic Assays
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Product Details
Features
- Determine the oxygen concentration that your cells are actually experiencing in culture
- Investigate the interplay between cellular oxygenation and metabolism
- Determine cellular oxygenation under hypoxic conditions and upon drug exposure
- Identify appropriate environmental oxygen concentration when conducting experiments where a defined intracellular oxygen concentration is crucial
- Simple 'mix-and-measure' protocol allows multiparametric analysis with a range of other kits, such as the Agilent pH-Xtra™ glycolysis assay and third-party assays for reactive oxygen species, mitochondrial membrane potential, and ATP
How It Works
Literature
- Application Notes
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Assessing the Impact of Drug Treatment on Cardiomyocyte Function
A demonstration of combining microelectrode-based iPS cardiomyocyte contractility measurements with microplate-based bioenergetics assessment to characterize cellular responses to drug treatment.
- Application Notes
- English
- 12 Mar 2021
- 1.01 MB
- Brochures
- Data Sheets
- Flyers
- Posters
Tools
Agilent Cell Analysis Publication Database
Search scientific publications by research area, cell type and more.
Seahorse XF Assay Learning Center
Up-to-date information on protocols and procedures for preparing, running, and analyzing an XF assay.
Support
- User Manuals
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Optimization of the Agilent MitoXpress Intra Intracellular Oxygen Assay
This guide contains instructions to maximize functionality of the MitoXpress Intra protocol template.
- User Manuals
- English
- 07 Feb 2023
- 1.19 MB
Mito Xpress Intra Intracellular Oxygen Assay
User guide for the MitoXpress Intra Assay kit. Includes workflow, plate reader-, storage conditions and signal optimization instructions.
- User Manuals
- English
- 04 Mar 2022
- 1.03 MB
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