Hydrophobic Interaction Chromatography (HIC) for Challenging Protein Separations
Hydrophobic interaction chromatography (HIC) is an invaluable tool for separating protein variants based on subtle differences in hydrophobicity. Unlike reversed-phase chromatography, which is performed under denaturing conditions, HIC is performed in aqueous eluents containing just salt and buffer, which keeps the protein in its native state. HIC columns address challenging molecules, including antibody drug conjugates (ADCs), monoclonal antibodies (mAbs), and other recombinant proteins.
