Endoglycosidases for glycoprotein and released glycan analysis
Endoglycosidases
Endoglycosidases release oligosaccharides from glycoproteins and some other substrates. These enzymes are useful for deglycosylating glycoproteins prior to analysis, or for releasing N-glycans for labeling and analysis. Useful in N-glycan analysis, Agilent offers PNGase F, an asparagine amidase that releases intact N-glycans. PNGase F cleaves between the innermost GlcNac of the N-glycan and the asparagine (Asn) residue of the peptide to which the N-glycan is linked
- Glycobiology Enzymes for N-Glycan Workflows
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Product Details
Features
- These products are most commonly used to remove glycans for analysis, or to obtain deglycosylated proteins for analysis.
- Notable among these these is PNGase F, an asparagine amidase that releases intact N-glycans by cleaving between the innermost GlcNAc of the N-glycan and the asparagine (Asn) residue of the peptide the N-glycan is linked to.
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Complete N-Glycan Workflow Solutions
Agilent offers tools and support for every step of your glycan sample preparation and analysis workflow.
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- Szabo Z, Thayer JR, Reusch D, Agroskin Y, Viner R, Rohrer J, Patil SP, Krawitzky M, Huhmer A, Avdalovic N, Khan SH, Liu Y, Pohl C. High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin. J Proteome Res. 2018 Apr 6;17(4):1559-1574.
- Küster B, Wheeler SF, Hunter AP, Dwek RA, Harvey DJ. Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography. Anal Biochem. 1997 Jul 15;250(1):82-101.
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- Reesink HL, Bonnevie ED, Liu S, Shurer CR, Hollander MJ, Bonassar LJ, Nixon AJ. Galectin-3 Binds to Lubricin and Reinforces the Lubricating Boundary Layer of Articular Cartilage. Sci Rep. 2016 May 9;6:25463.
- Chachadi VB, Bhat G, Cheng PW. Glycosyltransferases involved in the synthesis of MUC-associated metastasis-promoting selectin ligands. Glycobiology. 2015 Sep;25(9):963-75.
- Umemoto E, Tanaka T, Kanda H, Jin S, Tohya K, Otani K, Matsutani T, Matsumoto M, Ebisuno Y, Jang MH, Fukuda M, Hirata T, Miyasaka M. Nepmucin, a novel HEV sialomucin, mediates L-selectin-dependent lymphocyte rolling and promotes lymphocyte adhesion under flow. J Exp Med. 2006 Jun 12;203(6):1603-14.
- Terkelsen T, Haakensen VD, Saldova R, Gromov P, Hansen MK, Stöckmann H, Lingjaerde OC, Børresen-Dale AL, Papaleo E, Helland Å, Rudd PM, Gromova I. N-glycan signatures identified in tumor interstitial fluid and serum of breast cancer patients: association with tumor biology and clinical outcome. Mol Oncol. 2018 Jun;12(6):972-990
- Andrade-Silva D, Ashline D, Tran T, Lopes AS, Cardoso SRT, Reis MS, Zelanis A, Serrano SMT, Reinhold VN. Structures of N-Glycans of Bothrops venoms revealed as molecular signatures that contribute to venom phenotype in viperid snakes. Mol Cell Proteomics. 2018 May 1. pii: mcp.RA118.000748. doi: 10.1074/mcp.RA118.000748. [Epub ahead of print]
- Kim KK, Kim MK, Lim JH, Park HY, Lee ST. Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1287-93.
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