GlykoPrep Technology

The GlykoPrep platform streamlines N-glycan sample preparation. From purification to glycan release and labeling, the entire sample preparation workflow can be completed in as little as 3 hours. The cartridge-based platform supports traditional glycan labels such as 2-AB and APTS. Innovative high-performance dyes such as InstantPC are also supported. Your lab will enjoy improved efficiency and enhanced precision with GlykoPrep.

N-Glycan Sample Preparation Kits

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Features

The GlykoPrep platform supports traditional glycan labels such as 2-AB and APTS, as well as the first commercially available instant label for glycans, InstantAB, and the innovative InstantPC.
GlykoPrep is modular and can be integrated into any workflow. Kit components are also available individually as Digestion Module and dye-specific Labeling & Cleanup Modules, with optional AssayMAP PA50 module for purification of Fc-containing antibodies.
GlykoPrep is built on AssayMAP technology - microchromatography in a 96-well format. In the manual protocol, GlykoPrep is performed using centrifugation to move liquid through the cartridges (spin format). The workflow is automatable on the Agilent AssayMAP Bravo, see GlykoPrep-plus kit webpages for more detail.

Featured References

Publications

Szabo Z, Thayer JR, Reusch D, Agroskin Y, Viner R, Rohrer J, Patil SP, Krawitzky M, Huhmer A, Avdalovic N, Khan SH, Liu Y, Pohl C. High Performance Anion Exchange and Hydrophilic Interaction Liquid Chromatography Approaches for Comprehensive Mass Spectrometry-Based Characterization of the N-Glycome of a Recombinant Human Erythropoietin. J Proteome Res. 2018 Apr 6;17(4):1559-1574.
Szekrényes Á, Park SS, Cosgrave E, Jones A, Haxo T, Kimzey M, Pourkaveh S, Szabó Z, Sosic Z, Feng P, Sejwal P, Dent K, Michels D, Freckleton G, Qian J, Lancaster C, Duffy T, Schwartz M, Luo JK, van Dyck J, Leung PK, Olajos M, Kowle R, Gao K, Wang W, Wegstein J, Tep S, Domokos A, Váradi C, Guttman A. Multi-site N-Glycan mapping study 2: UHPLC. Electrophoresis. 2018 Apr;39(7):998-1005.
Szekrényes Á, Park SS, Santos M, Lew C, Jones A, Haxo T, Kimzey M, Pourkaveh S, Szabó Z, Sosic Z, Feng P, Váradi C, de l'Escaille F,Falmagne JB, Sejwal P, Niedringhaus T, Michels D, Freckleton G, Hamm M, Manuilov A, Schwartz M, Luo JK, van Dyck J, Leung PK, Olajos M, Gu Y, Gao K, Wang W, Wegstein J, Tep S, Guttman A. Multi-Site N-glycan mapping study 1: Capillary electrophoresis - laser induced fluorescence. MAbs. 2016 Jan 2;8(1):56-64.
Reusch D, Haberger M, Maier B, Maier M, Kloseck R, Zimmermann B, Hook M, Szabo Z, Tep S, Wegstein J, Alt N, Bulau P, Wuhrer M. Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles--part 1: separation-based methods. MAbs. 2015;7(1):167-79.
Magorivska I, Döncző B, Dumych T, Karmash A, Boichuk M, Hychka K, Mihalj M, Szabó M, Csánky E, Rech J, Guttman A, Vari SG, Bilyy R. Glycosylation of random IgG distinguishes seropositive and seronegative rheumatoid arthritis. Autoimmunity. 2018 May;51(3):111-117.
Turyan I, Hronowski S, Lyubarskaya Y. Comparison of two approaches for quantitative O-linked glycan analysis used in characterization of recombinant proteins. Anal Biochem. 2014 Feb 1;446:2836

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