AdvanceBio Gly-X Technology
The Agilent AdvanceBio Gly-X (formerly ProZyme) technology is a new generation of N-glycan sample preparation. Be ready for sample analysis faster with rapid (5-minute) enzymatic release of N-glycans and labeling with InstantDyes including the brightest in class InstantPC, or traditional reductive amination dyes. Innovative in-solution workflows combined with plate-based rapid cleanup enables sample prep in as little as 1 hour. Increase analytical sensitivity with a variety of dye options, while increasing sample throughput.
- Glycan Preparation
Product Details
Features
- Four dyes are currently available for the Gly-X platform:
- InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
- 2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
- InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Sample prep time: ~1 hour.
- APTS Express: APTS (8-aminopyrene-1,3,6-trisulfonic acid) is a negatively charged N-glycan label commonly used for capillary electrophoresis (CE) separations. Traditionally, labeling with APTS requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X APTS Express on-matrix labeling. Sample prep time: ~2.5 hours.
- InstantQ: a negatively charged N-glycan label for capillary electrophoresis (CE), designed for use with our Gly-Q N-glycan analysis platform. Sample prep time: ~1 hour.
Literature
- Application Notes
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Development of a Rapid 2-AB Sample Preparation Workflow for N-Glycans
The characterization of N-glycans is essential to the development of biotherapeutics.
- Application Notes
- English
- 08 Jan 2021
- 601.12 KB
Development of a Rapid APTS Sample Preparation Workflow for N-Glycans
Comparison of the fluorescence and MS response of Agilent InstantDyes using the Agilent Glykoprep Rapid N-glycan preparation system.
- Application Notes
- English
- 26 May 2020
- 778.30 KB
Streamlined Workflows for N-Glycan Analysis of Biotherapeutics with LC/FLD/MS
Streamlined Workflows for N-Glycan Analysis of Biotherapeutics Using Agilent AdvanceBio Gly-X InstantPC and 2-AB Express Sample Preparation with LC/FLD/MS
- Application Notes
- English
- 03 Dec 2019
- 959.04 KB
Preparation of Released N-Glycan Samples from Monoclonal Antibodies Using Agilent AdvanceBio Gly-X 2-AB Express for LC-Fluorescence Analysis
A method using the Agilent AdvanceBio Gly-X 2-AB Express kit and 2-AB labeling for the release of glycans with LC/FLD analysis.
- Application Notes
- English
- 17 Sep 2019
- 880.59 KB
High-Throughput CE N-Glycan Analysis for Clone Screening
Rapid N-glycan preparation and screening of biosimilar candidates by LC/FLD/MS and CE.
- Application Notes
- English
- 02 Aug 2019
- 715.67 KB
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AdvanceBio Gly-X Technology Frequently Asked Questions
Frequently Asked Questions for Gly-X Technology
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