AdvanceBio Gly-X Technology
- Glycan Preparation
Product Details
- Four dyes are currently available for the Gly-X platform:
- InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
- 2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
- InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Sample prep time: ~1 hour.
- APTS Express: APTS (8-aminopyrene-1,3,6-trisulfonic acid) is a negatively charged N-glycan label commonly used for capillary electrophoresis (CE) separations. Traditionally, labeling with APTS requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X APTS Express on-matrix labeling. Sample prep time: ~2.5 hours.
- InstantQ: a negatively charged N-glycan label for capillary electrophoresis (CE), designed for use with our Gly-Q N-glycan analysis platform. Sample prep time: ~1 hour.
- Application Notes
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Preparation of Released N-Glycan Samples from Monoclonal Antibodies Using Agilent AdvanceBio Gly-X 2-AB Express for LC-Fluorescence Analysis
A method using the Agilent AdvanceBio Gly-X 2-AB Express kit and 2-AB labeling for the release of glycans with LC/FLD analysis.
- Application Notes
- English
- 17 Sep 2019
- 880.59 KB
- Flyers
- Posters
- User Manuals
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Agilent Gly-X N-Glycan Rapid Release and Labeling with InstantAB Kit
The Agilent Gly-X N-Glycan Rapid Release and Labeling with InstantAB kit utilizes a novel in-solution enzymatic protein deglycosylation with N-Glycanase followed by fast labeling of released N-glycans with InstantAB dye.
- User Manuals
- English
- 02 Aug 2019
- 756.52 KB
Agilent AdvanceBio Gly-X N-Glycan Prep with InstantPC Kit, 96-ct
The Agilent Gly-X N-Glycan Rapid Release and Labeling with InstantPC kit utilizes a novel in-solution enzymatic protein deglycosylation with N-Glycanase followed by fast labeling of released N-glycans with InstantPC dye.
- User Manuals
- English
- 02 Aug 2019
- 1.01 MB
Agilent AdvanceBio Gly-X N-Glycan Prep with 2-AB Express Kit, 96-ct
The Agilent Gly-X N-Glycan Rapid Release and Labeling with 2-AB Express kit uses a novel in-solution enzymatic protein deglycosylation followed by fast labeling of released N-glycans with 2-AB dye.
- User Manuals
- English
- 02 Aug 2019
- 1.41 MB
Agilent AdvanceBio Gly-X N-Glycan Prep with InstantQ Kit, 96-ct
The Agilent Gly-X N-Glycan Rapid Release and Labeling with InstantQ kit utilizes a novel in-solution enzymatic protein deglycosylation with N-Glycanase followed by fast labeling of released N-glycans with InstantQ dye.
- User Manuals
- English
- 02 Aug 2019
- 1.22 MB
- Publications
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