Bio-Monolith Protein A and Protein G Affinity Columns
Affinity chromatography is one of the most effective ways to determinate the titer of monoclonal antibodies. Protein A and Protein G affinity chromatography columns absorb the IgG molecules onto the affinity resins which contain either immobilized protein A ligand or protein G ligand. Then, the remaining impurities and by-products from the fermentation broth can be removed. Elution of the purified monoclonal antibody and quantification by comparing the peak area to a calibration curve allows rapid measurement of the protein concentration.
Bio-Monolith Protein A and Protein G affinity columns are designed for analytical separation of all IgG subclasses (human and mouse). Delivering fast and accurate quantitation of mAb titer (sub 1 minute) and purification of small amounts of mAb for subsequent Critical Quality Attribute (CQA) analysis by another complementary technique, such as Aggregate Analysis or Charge Variant Analysis, they can easily be combined into a 2D-LC workflow.
Bio-Monolith Protein A and Protein G affinity chromatography columns are compatible with HPLC and UHPLC systems, including the Agilent 1100, 1200, 1260, and 1290 Bio-inert Quaternary LC Systems.