AdvanceBio SEC Columns with new sustainable packaging

AdvanceBio SEC HPLC Bio Columns

AdvanceBio SEC HPLC columns deliver accurate, precise quantitation for aggregate and fragment analysis for peptides, monoclonal antibodies, and larger next generation biotherapeutics. These innovative size exclusion chromatography columns were designed and engineered by Agilent to improve lab productivity by providing robust, reliable methods that eliminate sample re-analysis. Consistent results are achieved from column to column, batch to batch, and lab to lab, ensuring that methods can be transferred across departments and locations to put an end to uncertainty.

AdvanceBio SEC 2.7 µm columns are robust columns for routine SEC needs, with a unique hydrophilic coating to minimize secondary interactions between the sample and stationary phase. Even for sticky samples such as antibody-drug conjugates (ADCs), this column has minimized secondary interactions for accurate answers. Originally offered in two pore sizes, 300 Å and 130 Å, they are ideal for the sensitive, highly reproducible aggregate analysis of proteins and peptides.

The newest AdvanceBio SEC 2.7 µm columns offer larger 500 Å and 1000 Å pore sizes, developed and optimized for robust, high-resolution aggregate analysis of adeno-associated viruses (AAVs) and virus-like particles (VLPs).

AdvanceBio SEC 1.9 µm columns raise your SEC separations to the next level. The smaller particle size and hydrophilic chemistry results in high-efficiency, high-resolution separations. Stability up to 620 bar enables faster flow rates for high-throughput needs. The 200 Å pore size was specifically optimized for simultaneous separation of dimer, monomer, and fragment for thorough mAb characterization.

Aggregate & Fragment Analysis

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PL1180-5301	1 AdvanceBio SEC 300Å, 7.8 x 300 mm, 2.7 µm, HPLC column	7.8 mm	300 mm	2.7 µm	300 Å		1 Each	2,695.00 USD	Quantity
1 AdvanceBio SEC 300Å, 7.8 x 300 mm, 2.7 µm, HPLC column. A size exclusion chromatography (SEC) column for efficient aggregate analysis of proteins, including monoclonal antibodies. The resolving range is 5-1,250 kDa. Pressure Rating: 400 bar Molecular Weight Lower Limit: 5000 Da Molecular Weight Upper Limit: 1250000 Da Inner Diameter (ID): 7.8 mm Length: 300 mm Pore Size: 300 Å See all product information In Stock
PL1580-3302	AdvanceBio SEC 1000 Å, 2.7 µm, 4.6x150mm	4.6 mm	150 mm	2.7 µm	1000 Å	No	1 Each	2,898.00 USD	Quantity
AdvanceBio SEC 1000 Å, 2.7 µm, 4.6x150mm. A size exclusion column for aggregate analysis of large biological molecules such as VLPs and large oligonucleotides. Guard Column: No Pressure Rating: 400 bar Molecular Weight Lower Limit: 15000 Da Molecular Weight Upper Limit: 10 MDa Inner Diameter (ID): 4.6 mm Length: 150 mm Pore Size: 1000 Å See all product information In Stock
PL1580-5302	AdvanceBio SEC 1000 Å, 2.7 µm, 4.6x300mm	4.6 mm	300 mm	2.7 µm	1000 Å	No	1 Each	3,209.00 USD	Quantity
AdvanceBio SEC 1000 Å, 2.7 µm, 4.6x300mm. A size exclusion column for aggregate analysis of large biological molecules such as VLPs and large oligonucleotides. Guard Column: No Pressure Rating: 400 bar Molecular Weight Lower Limit: 15000 Da Molecular Weight Upper Limit: 10 MDa Inner Diameter (ID): 4.6 mm Length: 300 mm Pore Size: 1000 Å See all product information In Stock
PL1180-5302	AdvanceBio SEC 1000 Å, 2.7 µm, 7.8 x 300 mm	7.8 mm	300 mm	2.7 µm	1000 Å	No	1 Each	3,830.00 USD	Quantity
AdvanceBio SEC 1000 Å, 2.7 µm, 7.8 x 300 mm. Size exclusion column for aggregate analysis of large biological molecules, such as VLPs and large oligonucleotides. Guard Column: No Pressure Rating: 400 bar Molecular Weight Lower Limit: 15000 Da Molecular Weight Upper Limit: 10 MDa Inner Diameter (ID): 7.8 mm Length: 300 mm Pore Size: 1000 Å See all product information In Stock
PL1580-3250	AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 150 mm	4.6 mm	150 mm	1.9 µm	120 Å	No	1 Each	3,005.00 USD	Quantity
AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 150 mm. A size exclusion column designed to improve resolution and provide faster separation of protein and peptide aggregates and fragments by UHPLC. Resolving range is 1-80 kDa. Guard Column: No Pressure Rating: 620 bar Molecular Weight Lower Limit: 1000 Da Molecular Weight Upper Limit: 80000 Da Inner Diameter (ID): 4.6 mm Length: 150 mm Pore Size: 120 Å See all product information In Stock
PL1580-5250	AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 300 mm	4.6 mm	300 mm	1.9 µm	120 Å	No	1 Each	4,271.00 USD	Quantity
AdvanceBio SEC 120 Å column, 1.9 µm, 4.6 x 300 mm. A size exclusion column designed to improve resolution and provide faster separation of protein and peptide aggregates and fragments by UHPLC. Resolving range is 1-80 kDa. Guard Column: No Pressure Rating: 620 bar Molecular Weight Lower Limit: 1000 Da Molecular Weight Upper Limit: 80000 Da Inner Diameter (ID): 4.6 mm Length: 300 mm Pore Size: 120 Å See all product information In Stock
PL1580-1250	AdvanceBio SEC 120 Å Guard Column, 1.9 µm, 4.6 x 30 mm	4.6 mm	30 mm	1.9 µm	120 Å	Yes	1 Each	910.00 USD	Quantity
AdvanceBio SEC 120 Å Guard Column, 1.9 µm, 4.6 x 30 mm. Recommended to protect and extend the lifetime of a longer analytical SEC column. Guard Column: Yes Pressure Rating: 620 bar Molecular Weight Lower Limit: 1000 Da Molecular Weight Upper Limit: 80000 Da Inner Diameter (ID): 4.6 mm Length: 30 mm Pore Size: 120 Å See all product information In Stock
PL1980-3250PK	AdvanceBio SEC 120A 1.9um 2.1x150mm PEEK	2.1 mm	150 mm	1.9 µm	120 Å	No	1 Each	2,324.00 USD	Quantity
AdvanceBio SEC 120A 1.9um 2.1x150mm PEEK. A size exclusion column designed for higher resolution and faster separation of protein and peptide aggregates and fragments. PEEK-lined stainless steel hardware offers a metal-free sample flow path. Guard Column: No Pressure Rating: 620 bar Molecular Weight Lower Limit: 1000 Da Molecular Weight Upper Limit: 80000 Da Inner Diameter (ID): 2.1 mm Length: 150 mm Pore Size: 120 Å See all product information Est. ship date 16 October
PL1980-1250PK	AdvanceBio SEC 120A 1.9um 2.1x50mm PEEK	2.1 mm	50 mm	1.9 µm	120 Å	No	1 Each	2,147.00 USD	Quantity
AdvanceBio SEC 120A 1.9um 2.1x50mm PEEK. A size exclusion column designed for higher resolution and faster separation of protein and peptide aggregates and fragments. PEEK-lined stainless steel hardware offers a metal-free sample flow path. Guard Column: No Pressure Rating: 620 bar Molecular Weight Lower Limit: 1000 Da Molecular Weight Upper Limit: 80000 Da Inner Diameter (ID): 2.1 mm Length: 50 mm Pore Size: 120 Å See all product information Est. ship date 24 October
PL1580-3350	AdvanceBio SEC 130Å, 4.6 x 150 mm, 2.7 µm, LC column	4.6 mm	150 mm	2.7 µm	130 Å		1 Each	1,711.00 USD	Quantity
AdvanceBio SEC 130Å, 4.6 x 150 mm, 2.7 µm, LC column. A size exclusion column for efficient aggregate analysis of peptides and small proteins. The resolving range is 0.1-120 kDa. Pressure Rating: 400 bar Molecular Weight Lower Limit: 100 Da Molecular Weight Upper Limit: 120000 Da Inner Diameter (ID): 4.6 mm Length: 150 mm Pore Size: 130 Å See all product information In Stock
PL1580-5350	AdvanceBio SEC 130Å, 4.6 x 300 mm, 2.7 µm, LC column	4.6 mm	300 mm	2.7 µm	130 Å		1 Each	2,359.00 USD	Quantity
AdvanceBio SEC 130Å, 4.6 x 300 mm, 2.7 µm, LC column. A size exclusion column for efficient aggregate analysis of peptides and small proteins. The resolving range is 0.1-120 kDa. Pressure Rating: 400 bar Molecular Weight Lower Limit: 100 Da Molecular Weight Upper Limit: 120000 Da Inner Diameter (ID): 4.6 mm Length: 300 mm Pore Size: 130 Å See all product information In Stock
PL1580-1350	AdvanceBio SEC 130Å, 4.6 x 50 mm, 2.7 µm, HPLC Guard Column	4.6 mm	50 mm	2.7 µm	130 Å	Yes	1 Each	876.00 USD	Quantity
AdvanceBio SEC 130Å, 4.6 x 50 mm, 2.7 µm, HPLC Guard Column. Recommended to protect and extend the lifetime of a longer analytical SEC column. Guard Column: Yes Pressure Rating: 400 bar Molecular Weight Lower Limit: 100 Da Molecular Weight Upper Limit: 120000 Da Inner Diameter (ID): 4.6 mm Length: 50 mm Pore Size: 130 Å See all product information In Stock

1 - 12 of 32 results

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Product Details

Features

High-resolution, high-efficiency separation, and accurate quantitation for aggregate and fragment analysis of peptides, proteins, and now larger biotherapeutics, such as AAVs and VLPs
Reduced secondary interactions with biomolecules
Assured consistent SEC column performance to eliminate rework
Enhanced column lifetime and productivity to meet robust critical quality attribute (CQA) analysis
Sustainable column packaging uses 50% less material, is made of fully recyclable paper, and reduces shipping carbon footprint by 30%.

How It Works

How Does SEC Work?

Size exclusion chromatography (SEC) is based on the ability of sample components of different sizes to migrate in and out of pores on the stationary phase to different extents. Ideally, there is no intermolecular interaction between the sample and the stationary phase. Larger analytes are not able to travel as far into the pores as smaller analytes, so they spend less time in the pores and elute from the column earlier. Smaller analytes can more effectively enter the pores, so they spend more time there and elute from the column later.

Choosing the Right SEC Pore Size for Proteins

Determining the right pore size is possibly the most important aspect of choosing an SEC column. From there, you can choose dimensions based on your resolution, throughput, injection volume, and sensitivity needs. SEC separation is driven by differences in hydrodynamic radius between analytes, or size of the analytes in solution. For globular proteins, size in solution typically correlates well with molecular weight. The Agilent SEC column brochure provides pore size recommendations based on molecular weight or hydrodynamic radius.

View the Brochure

Choosing the Right SEC Pore Size for Single- or Double-stranded Oligonucleotides

Because globular proteins and oligos have very different shapes, the molecular weight to SEC pore size correlation developed for globular proteins cannot be used for oligos. More elongated structures mean oligos require larger pore sizes than the equivalent molecular weight proteins, and that they diffuse very slowly and resolution is improved by lower flow rates, as described in this application note.

Simplify the Biopharma HPLC Columns Search with Intuitive eSelector Tools

Use the Biopharma HPLC Column eSelector Tools to quickly and easily find the right Biopharma HPLC columns. Begin selecting your column by one of the following:

Your unique application area
Your separation mode
Another vendor's part number

Use the eSelector Tool

Applications

Monitor GLP-1 Oligomeric State and Aggregation with SEC

SEC is a valuable technique to monitor aggregation and changes in oligomeric state of peptide and protein therapeutics. GLP-1 peptides have a complex oligomeric state that is pH dependent. This application note illustrates SEC/UV and SEC/MS workflows to monitor changes in GLP-1 oligomeric state as a function of pH.

View the Application Note

AdvanceBio SEC 500 Å Columns for Aggregate Analysis of AAVs

Adeno-associated viruses (AAVs) are a popular vector for the development of new cell and gene therapies, and like any other biotherapeutic, aggregation is a critical quality attribute that must be monitored closely. AdvanceBio SEC 500 Å columns enable fast, robust, and high-resolution separations for accurate and reproducible aggregate analysis of AAVs.

View the Application Note

AdvanceBio SEC 1000 Å Columns for Aggregate Analysis of Virus-like Particles

Virus-like particles (VLPs) are self-assembled protein structures that resemble viruses on their exterior but contain no viral genetic material. Aggregate analysis is also a critical quality attribute for VLPs, which can range significantly in size from 20 to 150 nm, making it challenging to select the optimum technique before you even get to SEC pore size selection. AdvanceBio SEC 1000 Å columns can be used for VLPs and other biotherapeutic molecules up to ~80 nm diameter, like the example here of a ~50 nm VLP.

View the Application Note

High-Throughput mAb Aggregate Analysis with Sub 2 µm SEC Columns

Robust, higher-resolution separations enable faster flow rates and shorter methods, allowing you to get more done in a day. AdvanceBio SEC 1.9 µm columns show < 2% decrease in efficiency over 400 injections and are rated to 600 bar, making them robust for high-speed, high-throughput work. 150 mm length AdvanceBio SEC 1.9 µm columns (part number PL1580-3201) maintain high resolution enabling mAb aggregate analysis in as little as three minutes.

View the Application Note
View the Brochure

Videos

Upgrading Your Aggregate Analysis of Viral Vectors with Novel Wide Pore SEC Columns

00:47:02

Agilent AdvanceBio SEC columns

00:03:47

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AdvanceBio SEC Standards

Peptide and protein MW standards for calibration or performance checks by SEC.

Category: Aggregate & Fragment Analysis

NIST mAb Monoclonal Antibody Reference Standard

mAb reference standard for system suitability testing, establishing method, instrument, or site-to-site variability & troubleshooting mAb CQA

Category: Intact & Subunit Analysis

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